Continuous elution and chromatogram simulation:

Here you can view an animation of your compounds moving along the column.

You can also observe a depiction of what a detector (like a UV-spectrometer) sees at the output of the column. This is what we call a "Chromatogram" (click here to see more details about the approximations used in this app).

In the present application, all the compounds are considered to induce the same relative response in the used detector, e.g. they are supposed to have the same absorptivity in UV spectrum if a UV detector is used at the output of the column.

For the calculations, we used an approximated formula proposed by Martin & Synge to deduce the concentrations on the plates. This formula allows to get rid of the very long factorials calculations which are present in the exact formula.

Also, as in the table above, we considered that the volumes of the mobile phase and the stationary phase in the column are equal.
This is not necessarily true for all columns, and a different ratio will give a different chromatogram, because the ratio v_s/v_m changes the P constant (see the below section about the number of plates estimation).

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Before starting the simulation, some information about the column and the operating conditions are needed:

Column dead volume (mL): (What is it?)

The dead volume is the volume of the mobile phase inside the column. It can be calculated as follows:
Dead volume= Column volume X Stationary phase porosity
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Mobile phase flow rate (mL/min):

To go further: